| 产品编号 | bs-23361R-APC |
| 英文名称1 | Rabbit Anti-GCLM/APC Conjugated antibody |
| 中文名称 | APC标记的γ 谷氨酰半胱氨酸连接酶调节亚基抗体 |
| 别 名 | Glutamate-Cysteine Ligase Modifier Subunit; Gamma-Glutamylcysteine Synthetase Regulatory Subunit; Glutamate--Cysteine Ligase Modifier Subunit; Gamma-ECS Regulatory Subunit; GCS Light Chain; GLCLR; Glutamate-Cysteine Ligase (Gamma-Glutamylcysteine Synthetase), Regulatory (30.8kD); Glutamate-Cysteine Ligase Modifier Subunit Delta2 Alternative Splicing; Glutamate--Cysteine Ligase Regulatory Subunit; Glutamate-Cysteine Ligase Regulatory Protein; Glutamate-Cysteine Ligase, Modifier Subunit; Gamma-Glutamylcysteine Synthetase; GSC Light Chain; GSH0_HUMAN; |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 转录调节因子 合成与降解 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 31kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GCLM |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.05M PB, pH 7.5. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015] Function: This protein is involved in step 1 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate. Subunit: Heterodimer of a catalytic heavy chain and a regulatory light chain. Subcellular Location: Cytosol Tissue Specificity: In all tissues examined. Highest levels in skeletal muscle. Similarity: Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily. Database links: Entrez Gene: 2730 Human Entrez Gene: 14630 Mouse Omim: 601176 Human SwissProt: P48507 Human SwissProt: O09172 Mouse Unigene: 315562 Human Unigene: 292676 Mouse Unigene: 2460 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
