| 产品编号 | bs-8311R-Gold |
| 英文名称1 | Rabbit Anti-phospho-MYL12B (Thr19 + Ser20)/Gold Conjugated antibody |
| 中文名称 | 胶体金标记的磷酸化肌球蛋白调节轻链12B抗体 |
| 别 名 | Mylc2b (phospho T19 + S20); p-(phospho T19 +S20);ML12B_HUMAN; MLC-2; MLC-2a; MLC-B; MLC20; MRLC2; MYL12B; MYLC2B; Myosin light chain 12B regulatory; Myosin regulatory light chain 12B; myosin regulatory light chain 2; Myosin regulatory light chain 2-B; myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; Myosin regulatory light chain MRLC2; myosin, light chain 12B, regulatory; OTTHUMP00000162244; OTTHUMP00000165806; OTTHUMP00000165807; OTTHUMP00000165808; SHUJUN-1; smooth muscle isoform. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul（10nm 15nm 35nm） |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 细胞生物 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Danio) |
| 产品应用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 18kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human Mylc2b around the phosphorylation site of Thr19 + Ser20 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存条件 | Store at 2-8 ºC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 产品介绍 |
background: The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).[supplied by OMIM, Mar 2008] Function: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion. Tissue Specificity: Ubiquitously expressed in various hematopoietic cells. Post-translational modifications: Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is reduced following epigallocatechin-3-O-gallate treatment. Similarity: Contains 3 EF-hand domains. Database links: Entrez Gene: 103910 Human Entrez Gene: 67938 Mouse Omim: 609211 Human SwissProt: O14950 Human SwissProt: Q3THE2 Mouse Unigene: 190086 Human Unigene: 464472 Human Unigene: 261329 Mouse Unigene: 485011 Mouse Unigene: 214633 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
