产品编号 | bsm-33016M-Gold |
英文名称1 | Mouse Anti-Strep-Tag II/Gold Conjugated antibody |
中文名称 | 胶体金标记的Strep-Tag II(标签)单克隆抗体 |
别 名 | Strep; Strep Tag II; Strep.Tag; Strep-Tag-II; Strep-Tag-II. |
规格价格 | 100ul/2980元 购买 大包装/询价 |
说 明 书 | 100ul(10nm 15nm 35nm) |
产品类型 | 标签抗体 |
研究领域 | |
抗体来源 | Mouse |
克隆类型 | Monoclonal |
克 隆 号 | 9B11 |
交叉反应 | Recombinant protein |
产品应用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 状 | Lyophilized or Liquid |
浓 度 | 0.4mg/ml |
免 疫 原 | KLH conjugated synthetic peptide of Strep-Tag |
亚 型 | IgG |
纯化方法 | affinity purified by Protein G |
储 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
保存条件 | Store at 2-8 ºC for 3-6 months. Avoid repeated freeze/thaw cycles. |
产品介绍 |
background: The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |