产品编号 | bs-12789R-BF350 |
英文名称 | Rabbit Anti-ZP1/BF350 Conjugated antibody |
中文名称 | BF350标记的卵透明带糖蛋白1抗体 |
别 名 | MGC87693; Processed zona pellucida sperm-binding protein 1; zona pellucida glycoprotein 1 (sperm receptor); Zona pellucida glycoprotein 1; zona pellucida protein 1; Zp 1; Zp-1; Zp1; ZP1_HUMAN. |
规格价格 | 100ul/2980元 购买 大包装/询价 |
说 明 书 | 100ul |
研究领域 | 细胞生物 发育生物学 干细胞 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, (predicted: Mouse, Rat, Rabbit, ) |
产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 68kDa |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ZP1 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
产品介绍 |
background: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene ensures the structural integrity of the zona pellucida. Mutations in this gene are a cause of oocyte maturation defect and infertility. [provided by RefSeq, May 2014] Function: The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida. Subunit: Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers. Subcellular Location: Secreted > extracellular space > extracellular matrix and Cell membrane. Tissue Specificity: Oocytes. Post-translational modifications: Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida. O-glycosylated. Similarity: Belongs to the ZP domain family. ZPB subfamily. Contains 1 P-type (trefoil) domain. Contains 1 ZP domain. Database links: Entrez Gene: 22917 Human Omim: 195000 Human SwissProt: P60852 Human Unigene: 172130 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |