| 产品编号 | bs-19968R-Cy5.5 |
| 英文名称 | Rabbit Anti-PPP2R2D/Cy5.5 Conjugated antibody |
| 中文名称 | Cy5.5标记的蛋白磷酸酶2调节亚基2D抗体 |
| 别 名 | 2ABD_HUMAN; MDS026; PP2A subunit B isoform B55-delta; PP2A subunit B isoform delta; PP2A subunit B isoform PR55-delta; PP2A subunit B isoform R2-delta; PPP2R2D; Protein phosphatase 2 regulatory subunit B delta; Protein phosphatase 2 regulatory subunit B delta isoform; Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, (predicted: Human, Horse, ) |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 52kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PPP2R2D |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
Function: B regulatory subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. The activity of PP2A complexes containing PPP2R2D (PR55-delta) fluctuate during the cell cycle: the activity is high in interphase and low in mitosis. During mitosis, activity of PP2A is inhibited via interaction with phosphorylated ENSA and ARPP19 inhibitors. Within the PP2A complexes, the B regulatory subunits modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment (By similarity). Subunit: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with ENSA (when phosphorylated at 'Ser-67') and ARPP19 (when phosphorylated at 'Ser-62'), leading to inhibit PP2A activity (By similarity). Subcellular Location: Cytoplasm. Similarity: Belongs to the phosphatase 2A regulatory subunit B family. Contains 7 WD repeats. Database links: Entrez Gene: 423970 Chicken Entrez Gene: 55844 Human Entrez Gene: 52432 Mouse Entrez Gene: 379305 Xenopus laevis Entrez Gene: 448324 Xenopus tropicalis Entrez Gene: 327263 Zebrafish Omim: 613992 Human SwissProt: Q5ZIY5 Chicken SwissProt: Q4R8L3-2 Cynomolgus Monkey SwissProt: Q66LE6 Human SwissProt: Q925E7 Mouse SwissProt: Q5R4A2 Orangutan SwissProt: P63150 Rabbit SwissProt: Q7ZX64 Xenopus laevis SwissProt: Q6DIY3 Xenopus tropicalis SwissProt: Q6NY64 Zebrafish Unigene: 380372 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
