| 产品编号 | bs-8852R-Cy5 |
| 英文名称 | Rabbit Anti-d-myc/Cy5 Conjugated antibody |
| 中文名称 | Cy5标记的d-myc蛋白抗体 |
| 别 名 | dMyc1; MYC_DROME; Myc protein; d-Myc1; d-myc. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Firefly, fruit fly) |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 79kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from fruit fly d-myc |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Drosophila melanogaster is a proven and effective model for studying developmental and cellular processes common to higher eukaryotes. Approximately 13,600 genes have been elucidated from more than 120 megabases of euchromatin, and they are organized among the chromosomes 2, 3, 4, X and Y, with the Y chromosome being predominately heterochromatic (1). Drosophila genes can be categorized based on the type of protein they encode and are represented by six major classifications, which include intracellular signaling proteins, transmembrane proteins, RNA binding proteins, secreted factors, transcription regulators (basic helix-loop-helix, homeodomain containing, zinc finger containing, and chromatin associated) or other functional proteins (2). Many of the proteins in Drosophila are structurally and functionally similar across species, as are the pathways involved in transducing intracellular signaling. Among these proteins, myc (d-myc, dmyc1) is a transcription factor that links patterning signals to cell division by regulating events coordinating cellular growth and metabolism (3-7). Function: Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence CAC[GA]TG. Seems to activate the transcription of growth-related genes; required for cellular proliferation and growth. Inhibits the demethylase activity of Lid. Subunit: Heterodimer with another bHLH proteins. Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with Max. Interacts with lid. Part of a complex containing lid, dm and ash2. Component of a complex with pont and rept. Subcellular Location: Nucleus. Tissue Specificity: Low levels detected throughout embryo before cellular blastoderm formation, particularly concentrated in pole plasm. Zygotic expression detected during cellular blastoderm stage in endodermal anlagen of anterior and posterior midgut at both poles. After gastrulation, expression detected in invaginating ventral furrow of mesoderm. Continued expression in anterior and posterior midgut and mesoderm during germband extension. During late germ-band retraction, expression remains detectable in fusing midgut and presumed developing somatic musculature. Similarity: Contains 1 bHLH (basic helix-loop-helix) domain. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
