| 产品编号 | bs-17425R-PE-Cy7 |
| 英文名称1 | Rabbit Anti-Histone H2A.V/PE-Cy7 Conjugated antibody |
| 中文名称 | PE-Cy7标记的组蛋白H2家族A.V抗体 |
| 别 名 | H2A.F/Z; h2afv; H2AV; H2AV_HUMAN; Histone H2A.V. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 表观遗传学 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Zebrafish, Xenopus laevis) |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 14kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Histone H2A.V |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
Function: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. Subunit: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFV forms a heterodimer with H2B (By similarity). Subcellular Location: Nucleus. Chromosome. Post-translational modifications: Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression. Similarity: Belongs to the histone H2A family. Database links: Entrez Gene: 94239 Human Entrez Gene: 77605 Mouse Entrez Gene: 100328558 Rabbit Entrez Gene: 444541 Xenopus laevis Entrez Gene: 548932 Xenopus tropicalis Entrez Gene: 252913 Zebrafish SwissProt: Q71UI9 Human SwissProt: Q3THW5 Mouse SwissProt: O62695 Rabbit SwissProt: Q6GM74 Xenopus laevis SwissProt: Q5BJ65 Xenopus tropicalis SwissProt: Q71PD7 Zebrafish Unigene: 488189 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
