| 产品编号 | bs-16253R-APC |
| 英文名称 | Rabbit Anti-GLCE/APC Conjugated antibody |
| 中文名称 | APC标记的葡萄糖醛酸差向异构酶抗体 |
| 别 名 | Glucuronic Acid Epimerase; D glucuronyl C5 epimerase; GLCE; GLCE_HUMAN; Heparin/heparan sulfate:glucuronic acid C5 epimerase; HSEPI; KIAA0836. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 细胞生物 神经生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat, (predicted: Human, Mouse, Dog, Pig, Cow, Sheep, ) |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 70kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GLCE |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: GLCE (glucuronic acid epimerase), also known as HSEPI (heparin/heparan sulfate:glucuronic acid C5-epimerase) or D-glucuronyl C5-epimerase, is a single-pass type II membrane protein that is part of the golgi apparatus and, through its enzymatic activity, is essential for proper biological function of heparan sulphate (HS). GLCE epimerizes D-glucuronic acid into L-iduronic acid of HS, thus changing the specificity of HS and allowing it to bind to cytokines and growth factors. GLCE is a target of the beta-catenin–TCF4 transactivation complex; an essential component in the Wnt/APC/beta-catenin signaling pathway that is upregulated in colon carcinoma cells. The enzymatic activity of GLCE is enhanced by overexpression of beta-catenin–TCF4, suggesting a possible role for GLCE in the dysregulation of proper signaling pathways; a dysregulation that leads to the development of human epithelial tumors. Function: Glucuronic Acid Epimerase (GLCE) is responsible for epimerization of D-glucuronic acid (GlcA) to L-iduronic acid (IdoA) of Heparan sulfate (HS), which endows the nascent polysaccharide chain with the ability to bind growth factors and cytokines (Ghiselli and Agrawal, 2005 [PubMed 15853773]). Subunit: Interacts with HS2ST1 (By similarity). Subcellular Location: Golgi apparatus membrane; Single-pass type II membrane protein Similarity: Belongs to the D-glucuronyl C5-epimerase family. Database links: Entrez Gene: 26035 Human Entrez Gene: 93683 Mouse Omim: 612134 Human SwissProt: O94923 Human SwissProt: Q9EPS3 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
