| 产品编号 | bs-14405R-RBITC |
| 英文名称 | Rabbit Anti-DOCK9/RBITC Conjugated antibody |
| 中文名称 | 罗丹明（RBITC）标记的DOCK9蛋白抗体 |
| 别 名 | Cdc42 guanine nucleotide exchange factor zizimin 1; Dedicator of cytokinesis protein 9; DKFZp686C11110; DKFZp686D2047; DKFZp686N04132; DOCK 9; FLJ16744; FLJ44528; DOCK9_HUMAN; FLJ45282; FLJ45601; KIAA1058; KIAA1085; ZIZ1; Zizimin1. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 信号转导 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 236kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DOCK9 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: DOCK9 is a 2,069 amino acid protein that localizes to the intracytoplasmic membrane and contains one PH domain, one DHR-1 domain and one DHR-2 domain. Expressed in a variety of tissues with highest expression in placenta and heart and lower expression in lung, kidney, brain and skeletal muscle, Zizimin-1 functions as a guanine nucleotide-exchange factor (GEF) that specifically activates Cdc42 by exchanging bound GDP for free GTP. Four isoforms of Zizimin-1 exist due to alternative splicing events. The gene encoding Zizimin-1 maps to human chromosome 13, which houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome. Trisomy 13, also known as Patau syndrome, is deadly and the few who survive past one year suffer from permanent neurologic defects, difficulty eating and vulnerability to serious respiratory infections. Function: DOCK9 is a guanine nucleotide exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Its overexpression induces filopodia formation. Subunit: Homodimer (Probable). Interacts preferentially with nucleotide-depleted CDC42. Subcellular Location: Intracytoplasmic membrane Similarity: Belongs to the DOCK family. Contains 1 DHR-1 domain. Contains 1 DHR-2 domain. Contains 1 PH domain. Database links: Entrez Gene: 23348 Human Omim: 607325 Human SwissProt: Q9BZ29 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
