| 产品编号 | bs-13680R-APC |
| 英文名称1 | Rabbit Anti-UBXN2B/APC Conjugated antibody |
| 中文名称 | APC标记的UBXN2B蛋白抗体 |
| 别 名 | NSFL1 cofactor p37; p37; p97 cofactor p37; UBX domain containing protein 2B; UBX domain protein 2B; UBX domain-containing protein 2B; UBX2B_HUMAN; UBXN2B |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 信号转导 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Rabbit, ) |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 37kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human UBXN2B |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: The UBX (Ubiquitin regulatory X) domain is an 80 amino acid motif that is usually present on the carboxy-terminus of certain eukaryotic proteins. UBX domain-containing proteins (UBXD), such as FAF1, p33ING1 and D8S2298E, are typically involved in ubiquitin-related processes. UBXD proteins also constitute the largest family of VCP cofactors and are generally involved in substrate recruitment to VCP, as well as regulation of its activity. UBXD2B (UBX domain-containing protein 2B), also known as NSFL1 cofactor p37 and p97 cofactor p37, is a 331 amino acid protein that contains one UBX domain and one SEP domain. UBXN2B is required for ER and Golgi biogenesis and also plays a role in their maintenance during interphase, as well as their reassembly at the end of mitosis. Through interaction with VCP, UBXN2B forms a complex that has membrane fusion activity. Function: Adapter protein required for Golgi and endoplasmic reticulum biogenesis. Involved in Golgi and endoplasmic reticulum maintenance during interphase and in their reassembly at the end of mitosis. The complex formed with VCP has membrane fusion activity; membrane fusion activity requires USO1-GOLGA2 tethering and BET1L. VCPIP1 is also required, but not its deubiquitinating activity. Subunit: Interacts with VCP. Does not bind ubiquitin (By similarity). Subcellular Location: Nucleus. Cytoplasm; cytosol. Endoplasmic reticulum. Golgi apparatus. Similarity: Belongs to the NSFL1C family. Contains 1 SEP domain. Contains 1 UBX domain. Database links: Entrez Gene: 137886 Human Omim: 610686 Human SwissProt: Q14CS0 Human Unigene: 155572 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
