| 产品编号 | bs-13609R-HRP |
| 英文名称 | Rabbit Anti-DAZ4/HRP Conjugated antibody |
| 中文名称 | 辣根过氧化物酶标记的无精症缺失基因4抗体 |
| 别 名 | DAZ4; Deleted in azoospermia 4; Deleted in azoospermia protein 4; DAZ4_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 发育生物学 干细胞 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 65kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DAZ4 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Spermatogenesis is the process by which male spermatogonia develop into mature spermatozoa. DAZ (deleted in azoospermia) are RNA-binding proteins that play an essential role in spermatogenesis. DAZ proteins influence the first stages of spermatogenesis and the maintenance of germ cell populations. DAZ proteins (DAZ1, DAZ2, DAZ3, DAZ4 and DAZ5) are encoded by separate genes on chromosome Y, each of which contain an AZFc domain in their coding region. DAZ proteins localize to the nucleus of spermatogonia, but relocate to the cytoplasm during meiosis. DAZ proteins contain an RRM (RNA recognition motif) domain that may regulate mRNA translation by binding to the 3’UTR. Deletions in the genes encoding DAZ proteins may cause azoospermia or oligospermia which can lead to male infertility. DAZ4 (deleted in azoospermia 4), also known as pDP1680 or pDP1681, is a 579 amino acid testis specific protein that contains nine DAZ-like domains and two RNA recognition motifs (RRM). DAZ4 exists as two alternatively spliced isoforms. Function: Deleted in azoospermia 4 is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that plays an essential role in spermatogenesis. It may act by binding to the 3'-UTR of mRNAs and regulating their translation. Subcellular Location: Cytoplasm. Nucleus. Similarity: Belongs to the RRM DAZ family. Contains 9 DAZ-like domains. Contains 2 RRM (RNA recognition motif) domains. Database links: Entrez Gene: 57135 Human Omim: 400003 Human SwissProt: Q86SG3 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
