| 产品编号 | bs-15459R-Gold |
| 英文名称 | Rabbit Anti-HERC1 /Gold Conjugated antibody |
| 中文名称 | 胶体金标记的鸟苷酸释放因子p532蛋白抗体 |
| 别 名 | Guanine nucleotide exchange factor p532; Hect (homologous to the E6 AP (UBE3A) carboxyl terminus) domain and RCC1 (CHC1) like domain (RLD) 1; HECT and RLD domain containing E3 ubiquitin protein ligase family member 1; HECT domain and RCC1 like domain containing protein 1; p532; p619; Probable E3 ubiquitin-protein ligase HERC1; HERC1_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul（10nm 15nm 35nm） |
| 研究领域 | 细胞生物 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep, ) |
| 产品应用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 532kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HERC1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存条件 | Store at 2-8 ºC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 产品介绍 |
background: HERC1 is a stimulates guanine nucleotide exchange on ARF1 and Rab proteins. This protein may be involved in membrane transport processes. Function: Involved in membrane trafficking via some guanine nucleotide exchange factor (GEF) activity and its ability to bind clathrin. Acts as a GEF for Arf and Rab, by exchanging bound GDP for free GTP. Binds phosphatidylinositol 4,5-bisphosphate, which is required for GEF activity. May also act as a E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Subunit: Interacts with TSC2; interaction is inhibited by TSC1. Interacts with PKM, ARF1 and ARF6. Forms a ternary complex with clathrin heavy chain (CLTC) and HSPA1A. Subcellular Location: Membrane; Peripheral membrane protein. Cytoplasm, cytosol. Golgi apparatus. Note=Recruited onto actin-rich surface protrusions. Tissue Specificity: Widely expressed. Similarity: Contains 1 B30.2/SPRY domain. Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. Contains 14 RCC1 repeats. Contains 11 WD repeats. Database links: Entrez Gene: 8925 Human Entrez Gene: 235439 Mouse Omim: 605109 Human SwissProt: Q15751 Human Unigene: 210385 Human Unigene: 244179 Mouse Unigene: 232088 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
