产品编号 | bs-13496R-PE-Cy7 |
英文名称1 | Rabbit Anti-GPAA1/PE-Cy7 Conjugated antibody |
中文名称 | PE-Cy7标记的糖基磷脂酰肌醇锚连接蛋白1抗体 |
别 名 | Anchor attachment protein 1 (Gaa1p, yeast) homolog; GAA1; GAA1; GAA1; GAA1 protein homolog; Glycophosphatidylinositol anchor attachment 1; Glycosylphosphatidylinositol anchor attachment 1 protein; Glycosylphosphatidylinositol anchor attachment 1 protein; Glycosylphosphatidylinositol anchor attachment 1 protein; Glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast); GPAA 1; GPAA1; GPAA1_HUMAN; GPAA1P anchor attachment protein 1 homolog; GPI anchor attachment protein 1; GPI anchor attachment protein 1; GPI anchor attachment protein 1; GPI transamidase subunit; hGAA1. |
规格价格 | 100ul/2980元 购买 大包装/询价 |
说 明 书 | 100ul |
研究领域 | 细胞生物 细胞表面分子 细胞类型标志物 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Human, Mouse, Rat, Pig, Cow, Rabbit, Sheep, ) |
产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 68kDa |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human GPAA1 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
产品介绍 |
background: Glycosylphosphatidylinositol (GPI) acts as a membrane anchor for cell surface proteins. Glycosylphosphatidylinositol anchor attachment 1 protein (GPAA1), also designated GPI anchor attachment protein 1 or GAA1 protein homolog, is a membrane protein localized to the endoplasmic reticulum which is involved in GPI-anchor biosynthesis. GPAA1 is crucial for GPI-anchoring of precursor proteins and catalyzes the attachment of GPI to proteins containing a C-terminal GPR attachment signal. GAA1 contains an N-terminal signal sequence, one cAMP- and cGMP-dependent protein kinase phosphorylation site, two potential N-glycosylation sites, one leucine zipper pattern and eight putative transmembrane domains. GPAA1 is ubiquitously expressed and shows higher levels of expression in fetal tissues than in adult tissues. Function: Essential for GPI-anchoring of precursor proteins but not for GPI synthesis. Acts before or during formation of the carbonyl intermediate. Subunit: Forms a complex with PIGK/GPI8, PIGT, PIGU and PIGS. Subcellular Location: Endoplasmic reticulum membrane. Tissue Specificity: Ubiquitously expressed in fetal and adult tissues. Expressed at higher levels in fetal tissues than adult tissues. Database links: UniProtKB/Swiss-Prot: O43292.3 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |