| 产品编号 | bs-12349R-Cy7 |
| 英文名称 | Anti-ASCL2/Cy7 |
| 中文名称 | Cy7标记的ASCL2蛋白抗体 |
| 别 名 | Achaete scute complex like 2; Achaete scute homolog 2; achaete-scute complex-like 2; Achaete-scute homolog 2; ASCL2; ASCL2_HUMAN; ASH-2; Ash2; bHLHa45; Class A basic helix-loop-helix protein 45; HASH2; Mash2. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 发育生物学 信号转导 干细胞 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, ) |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 22kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Human ASCL2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Members of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes, both of which work together to activate DNA transcription. Class A proteins include the ubiquitously expressed E-box binding factors, namely E2A, ITF-2 and HEB, while class B proteins, such as MyoD, myogenin and Neuro D (BETA2), are transiently expressed and exhibit a much more limited tissue distribution. Working in opposition to these positively acting factors are a specialized group of basic helix-loop-helix (bHLH) transcription factors that function as dominant negative regulators and are involved in cell lineage determination and differentiation. ASCL2 is a 193 amino acid protein that localizes to the nucleus and contains one bHLH domain. Expressed in developing placental tissue, ASCL2 binds to DNA and functions as a transcriptional regulator that is involved in the maturation of neuronal precursors in the peripheral and central nervous systems. Function: AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system. Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Subcellular Location: Nucleus. Tissue Specificity: Expressed specifically in the extravillous trophoblasts of the developing placenta. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. Database links: Entrez Gene: 430 Human Entrez Gene: 17173 Mouse SwissProt: Q99929 Human SwissProt: O35885 Mouse Unigene: 152475 Human Unigene: 196417 Mouse Unigene: 10486 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
