| 产品编号 | bs-12305R-Bio |
| 英文名称 | Rabbit Anti-USE1/Biotin Conjugated antibody |
| 中文名称 | 生物素标记的囊泡转运蛋白Use1抗体 |
| 别 名 | 2010315L10Rik; 5730403H22Rik; AV002165; D12; Ed2; Embryonic development factor 2; MDS032; P31; Protein D12; Protein p31; putative MAPK activating protein PM26; Putative MAPK-activating protein PM26; Q-snare; RGD1306660; SLT1; SNARE-like tail-anchored protein 1 homolog; Unconventional SNARE in the ER 1 homolog (S. cerevisiae); Unconventional SNARE in the ER 1 homolog; Use1; USE1 like protein; Use1 unconventional SNARE in the ER 1 homolog (S. cerevisiae); USE1-like protein; USE1_HUMAN; USE1L; Vesicle transport protein USE1. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 发育生物学 信号转导 干细胞 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 29kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human USE1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈. |
| 产品介绍 |
background: In eukaryotic cells, the Golgi apparatus receives newly synthesized proteins from the endoplasmic reticulum (ER) and, after covalent modification, delivers them to their destination in the cell. For membrane-directed proteins this process is believed to be carried out via vesicular transport. Correct vesicular transport is determined by specific pairing of vesicle-associated SNAREs (v-SNAREs) with those on the target membrane (t-SNAREs). Unconventional SNARE in the ER 1, also known as USE1 or protein p31, is a 259 amino acid t-SNARE that forms a larger complex with ZW10, RINT-1 and Syntaxin 18. Upon Mg2+-AP treatment in the presence of NSF and ?SNAP, ZW10, RINT-1 and USE1 dissociate from Syntaxin 18. USE1 is a single-pass type IV membrane protein that is localized to the endoplasmic reticulum membrane. Three named isoforms exist for USE1 as a result of alternative splicing events. Function: SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Subunit: Component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B. Interacts directly with STX18. Subcellular Location: Endoplasmic reticulum membrane. Similarity: Belongs to the USE1 family. Database links: Entrez Gene: 512890 Cow Entrez Gene: 55850 Human Entrez Gene: 67023 Mouse Omim: 610675 Human SwissProt: Q9NZ43 Human SwissProt: Q9CQ56 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
