| 产品编号 | bs-5685R-HRP |
| 英文名称 | Rabbit Anti-phospho-RUNX2 (Ser451)/HRP Conjugated antibody |
| 中文名称 | 辣根过氧化物酶标记的磷酸化成骨特异性转录因子抗体 |
| 别 名 | RUNX2(phospho S451); Runt-related Transcription Factor 2; CBF alpha 1; CBFA1; CCD; CCD1; Cleidocranial dysplasia 1; Core binding factor; Core binding factor runt domain alpha subunit 1; Core binding factor subunit alpha 1; MGC120023; Oncogene AML 3; OSF 2; OSF2; Osteoblast specific transcription factor 2; OTTHUMP00000016533; PEA2 alpha A; PEA2aA; PEBP2 alpha A; PEBP2A1; PEBP2A2; PEBP2aA1; Polyomavirus enhancer binding protein 2 alpha A subunit; Runt domain; Runt related transcription factor 2; SL3 3 enhancer factor 1 alpha A subunit; SL3/AKV core binding factor alpha A subunit. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 免疫学 干细胞 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Chicken, Dog, Pig, Cow, Horse, ) |
| 产品应用 | ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 67kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human RUNX2 around the phosphorylation site of Ser451 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation. Subunit: Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 (By similarity). The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with G22P1 (Ku70) and XRCC5 (Ku80). Interacts with MYST3 and MYST4. Subcellular Location: Nucleus. Tissue Specificity: Specifically expressed in osteoblasts. Similarity: Contains 1 Runt domain. Database links: Entrez Gene: 860 Human Entrez Gene: 12393 Mouse Omim: 600211 Human SwissProt: Q13950 Human SwissProt: Q9XSB7 Horse SwissProt: Q08775 Mouse Unigene: 535845 Human Unigene: 391013 Mouse Unigene: 391017 Mouse Unigene: 214214 Rat Unigene: 83672 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. RUNX2又称：Cbfα1(Core-binding factor, alpha 3 subunit) 是新发现的一类调控间充质干细胞向成骨方向分化的特异性转录因子,参与骨形成,骨骼生长和发育的一类重要细胞,它起源于多能间充质干细胞,是间充质干细胞在体内的各种调控因素的调节下发育而成的。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
