| 产品编号 | bs-5159R-BF350 |
| 英文名称1 | Rabbit Anti-MYLPF/BF350 Conjugated antibody |
| 中文名称 | BF350标记的快速骨骼肌肌球蛋白轻链2抗体 |
| 别 名 | 2410014J02Rik; DKFZp779C0757; DTNB; Fast skeletal myosin light chain 2; G2; HUMMLC2B; MGC13450; MLC 2; MRLC2; MYL11; MLC2B; MLC2F; MLRS_HUMAN; MYLPF; Myosin light polypeptide 2 alkali; Myosin regulatory light chain 2, skeletal muscle isoform. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 免疫学 信号转导 细胞骨架 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 19kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Fast skeletal myosin light chain 2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional Myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of Myosin have been cloned: smooth muscle Myosins, striated muscle Myosins and non-muscle Myosins . Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain (MLC) by Myosin light chain kinase. Myosin heavy chains, which are encoded by the MYH gene family, contain Actin-activated ATPase activity which generates the motor function of Myosin. Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chains are differentially expressed depending on the functional activity of the muscle. Subunit: Myosin is a hexamer of 2 heavy chains and 4 light chains. Tissue Specificity: Expressed in fetal and adult skeletal muscle. Similarity: Contains 3 EF-hand domains. Database links: Entrez Gene: 29895 Human Entrez Gene: 396470 Chicken Entrez Gene: 4632 Human Entrez Gene: 17901 Mouse Entrez Gene: 17907 Mouse Omim: 160780 Human SwissProt: P02609 Chicken SwissProt: P05976 Human SwissProt: P06741 Human SwissProt: Q14324 Human SwissProt: P05978 Mouse SwissProt: P97457 Mouse SwissProt: P02602 Rabbit SwissProt: P02603 Rabbit Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
