| 产品编号 | bs-9719R-PE-Cy5 |
| 英文名称 | Anti-PDE4A8/PE-Cy5 |
| 中文名称 | PE-Cy5标记的磷酸二酯酶4A8抗体 |
| 别 名 | 2EL; DPDE2; PDE4; PDE46; PDE4A; Phosphodiesterase 4A; Phosphodiesterse Type 4A8; PDE4A_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 信号转导 细胞周期蛋白 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep, ) |
| 产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 98kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PDE4A8 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: The protein encoded by this gene belongs to the cyclicnucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. ThisPDE hydrolyzes the second messenger, cAMP, which is a regulator andmediator of a number of cellular responses to extracellularsignals. Thus, by regulating the cellular concentration of cAMP,this protein plays a key role in many important physiologicalprocesses. Alternatively spliced transcript variants encodingdifferent isoforms have been described for this gene.[provided byRefSeq, Jul 2011]. Function: Hydrolyzes the second messenger cAMP, which is a keyregulator of many important physiological processes. Subunit: Isoform 1 interacts with LYN. Isoform 2 and isoform 6interact weakly with LYN. Isoform 1, isoform 2 and isoform 6interact with ARRB2. Subcellular Location: Isoform 1: Cytoplasm, perinuclear region. Tissue Specificity: Isoform 1 is widely expressed. Isoform 2 isabundant in liver, stomach, testis, thyroid and adrenal glands. Itis also found in placenta, kidney, pancreas, ovary, uterus, skin,monocytes, mast cells, macrophages, as well as in bronchial smoothmuscle. Isoform 6 is expressed at high levels in the heart andsmall intestine. It is also found in the brain, kidney, spleen,colon, salivary gland, ovary and peripheral blood lymphocytes.Isoform 7 is expressed predominantly in skeletal muscle and brainand at lower levels in the testis. Isoform 7 is expressed in thebrain. Found in specific neuronal subpopulations in cortex, spinalcord and cerebellum (at protein level). Post-translational modifications: Phosphorylation by MAPKAPK2 its activation through PKAphosphorylation (By similarity). Phosphorylated at Ser-686 andSer-688 when expressed in S.frugiperda cells. Isoform 2 and isoform7 are activated by phosphorylation at Ser-119 and Ser-123respectively by PKA. Proteolytically cleaved by caspase-3. Similarity: Belongs to the cyclic nucleotide phosphodiesterasefamily. PDE4 subfamily. Database links: Entrez Gene: 5141 Human SwissProt: P27815 Human Unigene: 89901 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
