| 产品编号 | bs-9234R-BF594 |
| 英文名称1 | Rabbit Anti-CREG1/BF594 Conjugated antibody |
| 中文名称 | BF594标记的E1A激活基因刺激蛋白1抗体 |
| 别 名 | Cellular repressor of E1A stimulated genes 1; Cellular repressor of E1A-stimulated genes 1; CREG; CREG1; CREG1_HUMAN; Protein CREG1. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 免疫学 信号转导 细胞周期蛋白 转录调节因子 细胞分化 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 21kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CREG1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. CREG (cellular repressor of E1A-stimulated genes) is a cellular protein that antagonizes transcriptional activation and cellular transformation by E1A. CREG was initially isolated in a yeast two-hybrid screen due to its interaction with the TATA-binding protein, TBP. Binding sites for E2F, a key transcriptional reg-ulator of cell cycle progression, are required for repression of the adeno-virus E2 promoter by CREG, and CREG was shown to inhibit activation by E2F. CREG is broadly expressed in adult tissues and is regulated during embryonic development. CREG is a secreted glycoprotein which enhances differentiation of mouse embryonic stem cells and human NTERA-2 cells. CREG activity may contribute to the transcriptional control of cell growth and differentiation. Function: May contribute to the transcriptional control of cell growth and differentiation. Antagonizes transcriptional activation and cellular transformation by the adenovirus E1A protein. The transcriptional control activity of cell growth requires interaction with IGF2R. Subunit: Homodimer. Interacts with IGF2R; the interaction is dependent on glycosylation. Subcellular Location: Secreted. Post-translational modifications: N-glycosylated. Similarity: Belongs to the CREG family. Database links: Entrez Gene: 8804 Human SwissProt: O75629 Human Unigene: 5710 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
