| 产品编号 | bs-0054R-HRP |
| 英文名称 | Rabbit Anti-GDNF Receptor alpha 2/HRP Conjugated antibody |
| 中文名称 | 辣根过氧化物酶标记的胶质细胞系源性神经营养因子受体α2抗体 |
| 别 名 | GDNF family receptor alpha-2; GDNF Receptor α2; GDNF receptor beta; GDNFR beta; GDNFR-beta; GDNFRB; GFR alpha 2; GFRA 2; GFRA2; GFR alpha-2; Glial cell line derived neurotrophic factor family receptor alpha2b; Glial cell line derived neurotrophic factor receptor beta; Neurturin receptor alpha; NRTNR alpha; NRTNR-alpha; NTNR alpha; NTNR-alpha; NTNRA; PI linked cell surface accessory protein; RET ligand 2; RETL 2; RETL2; TGF beta related neurotrophic factor receptor 2; TRN receptor GPI anchored; TRNR 2; TRNR2; GFRA2_HUMAN; GDNF family receptor alpha-2; GDNF receptor alpha-2; Short=GDNFR-alpha-2; GFR-alpha-2; Neurturin receptor alpha; TGF-beta-related neurotrophic factor receptor 2. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 发育生物学 神经生物学 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Chicken, Dog, Cow, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 47kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GDNF Receptor alpha 2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. Multiple transcript variants encoding different isoforms have been found for this gene. Members of the glial cell line-derived neurotrophic factor(GDNF) family, including GDNF and neurturin (NTN),play key roles in the control of vertebrate neuron survivaland differentiation. Physiological responses to NTN requirethe presence of a novel glycosylphosphadidylinositollinked protein NTNRa, which is a cell surface receptor forNTN. The cDNAs encoding NTNRa from human, rat,chicken, and mouse have been cloned recently. NTNRa was also termed GDNFRb, Ret ligand 2 (RETL2)or TGF-b-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRa-2 recently. GFRa-2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF. Thus, NTN, GFRa-2, and the Ret PTK form a complex to transduce NTN signal andto mediate NTN function. Function: Receptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Tissue Specificity: Isoform 1 is found in both brain and placenta. Similarity: Belongs to the GDNFR family. Database links: Entrez Gene: 2675 Human Entrez Gene: 14586 Mouse Omim: 601956 Human SwissProt: O00451 Human SwissProt: O08842 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 神经生物学相关蛋白（Neurobiology） 胶质细胞源性神经营养因子(GDNF)是TGF-β家族的一个新亚族，该亚族还有新发现的三个成员：Neurturin(NTN)、Persephin(PSP)和Artemin(ART)，它们均不程度地对多巴胺神经元、脊髓前角运动神经元、背根神经节、颈上神经节以及肾等非神经元细胞都具有生长促进作用。研究表明胶质细胞系源性神经营养因子,主要用于PD的研究。 GDNF的受体是多成分的复合物，它是由固定于质膜外层的糖基磷脂酰肌醇(GPI)被称为GDNFRα和酪氨酸激酶RET蛋白组成。GDNFRα为其配体结合亚单位，而RET则是GDNF的功能性受体亚单位，即GDNF特异地结合于其受体的GDNFRα上，再激活RET的酪氨酸磷酸化传导其信号，影响细胞的活性，发挥其生物学效应。目前，又克隆出了人与大鼠的GDNFRβ序列，与GDNPRα具有47％的同源性。GDNFRβ mRNA在脊髓、DRG和发育中的周围神经上也均有表达。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
