| 产品编号 | bs-1039R-AP |
| 英文名称 | Rabbit Anti-Tenascin C/AP Conjugated antibody |
| 中文名称 | 碱性磷酸酶（AP）标记的细胞粘合素(固生蛋白)抗体 |
| 别 名 | Tn-C; Cytotactin; Glioma associated extracellular matrix antigen; GMEM; GP 150 225; Hexabrachion; HXB ; JI; Myotendinous antigen; Neuronectin; Tenascin C; TenascinC; TN; TN C; TNC; TENA_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, (predicted: Human, Pig, ) |
| 产品应用 | IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 239kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Tn-C |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Tenascin, also known as hexabrachion and cytotactin, is an extracellular matrix protein with a spatially and temporally restricted tissue distribution. It is a hexameric, multidomain protein with disulfide linked subunits of 190 to 240 kD, originally characterized as 'myotendinous antigen.' In the embryo it is present in dense mesenchyme surrounding developing epithelia and in developing cartilage and bone. In the adult, tenascin remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle. Function: Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. Subunit: Homohexamer; disulfide-linked. A homotrimer may be formed in the triple coiled-coil region and may be stabilized by disulfide rings at both ends. Two of such half-hexabrachions may be disulfide linked within the central globule. Interacts with CSPG4. Subcellular Location: Secreted, extracellular space, extracellular matrix. Similarity: Belongs to the tenascin family. Contains 15 EGF-like domains. Contains 1 fibrinogen C-terminal domain. Contains 15 fibronectin type-III domains. Database links: Entrez Gene: 3371 Human Entrez Gene: 21923 Mouse Omim: 187380 Human SwissProt: P24821 Human SwissProt: Q80YX1 Mouse Unigene: 143250 Human Unigene: 454219 Mouse Unigene: 980 Mouse Unigene: 12723 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Tenascin-C(TN-C)是重要的细胞外基质(ECM)成分之一,在组织器官的发生发育中发挥着极其重要的作用;其表达具有复杂的时空分布,并受多种因素(细胞因子、激素等)调节.目前主要用于消化系统肿瘤方面的研究。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
