| 产品编号 | bs-7937R-Gold |
| 英文名称 | Rabbit Anti-NIFK/Gold Conjugated antibody |
| 中文名称 | 胶体金标记的NIFK蛋白抗体 |
| 别 名 | hNIFK; MKI67 FHA Domain Interacting Nucleolar Phosphoprotein; MKI67IP; NOPP34; Nucleolar phosphoprotein Nopp34; Nucleolar protein interacting with the FHA domain of pKI 67; pKi67; MK67I_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul（10nm 15nm 35nm） |
| 研究领域 | 肿瘤 细胞生物 细胞周期蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat, (predicted: Human, Mouse, Dog, Rabbit, ) |
| 产品应用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 34kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NIFK |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存条件 | Store at 2-8 ºC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 产品介绍 |
background: This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009]. Subunit: Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis. Subcellular Location: Nucleus, nucleolus. Chromosome. Note=Localizes to mitotic chromosomes in conjunction with MKI67. Post-translational modifications: Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding. Similarity: Contains 1 RRM (RNA recognition motif) domain. Database links: Entrez Gene: 84365 Human Entrez Gene: 67949 Mouse Omim: 611970 Human SwissProt: Q8TB66 Human SwissProt: Q9BYG3 Human SwissProt: Q91VE6 Mouse Unigene: 367842 Human Unigene: 280311 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
