| 产品编号 | bs-7861R-PE-Cy5 |
| 英文名称1 | Rabbit Anti-EME1/PE-Cy5 Conjugated antibody |
| 中文名称 | PE-Cy5标记的减数分裂内切酶EME1抗体 |
| 别 名 | Crossover junction endonuclease EME1; Eme1 essential meiotic endonuclease 1 homolog 1 (S. pombe); EME1, S. pombe, homolog of, 1; essential meiotic endonuclease 1 homolog 1 (S pombe); Essential Meiotic Endonuclease 1 Homolog 1; essential meiotic endonuclease 1 homolog 2; Essential meiotic endonuclease 1, S. pombe, homolog of, 1; hMMS4; homolog of yeast EME1 endonuclease; MMS4; MMS4 homolog; MMS4L; EME1_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 发育生物学 干细胞 细胞周期蛋白 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Dog, Rabbit, Sheep, ) |
| 产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 63kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EME1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: EME1 complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein (MUS81) to form an endonuclease complex which cleaves branched DNA structures, especially those arising during stalled DNA replication. The protein may be involved in repairing DNA damage and in maintaining genomic stability. It interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. Alternative splicing results in multiple transcript variants. Function: Interacts with MUS81 to form a DNA structure-specificendonuclease with substrate preference for branched DNA structureswith a 5'-end at the branch nick. Typical substrates include3'-flap structures, replication forks and nicked Hollidayjunctions. May be required in mitosis for the processing of stalledor collapsed replication forks. Subunit: May self-associate. Interacts with MUS81. Interacts withERCC4 and FANCM. Subcellular Location: Nucleus, nucleolus. Note=Recruited toregions of DNA damage in S-phase cells. Similarity: Belongs to the EME1/MMS4 family. Database links: UniProtKB/Swiss-Prot: Q96AY2.2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
