| 产品编号 | bs-7844R-Gold |
| 英文名称 | Rabbit Anti-GSK3B interacting protein/Ninein/Gold Conjugated antibody |
| 中文名称 | 胶体金标记的GSK3B相互作用蛋白抗体 |
| 别 名 | Centrosome Marker; Centrosome Marker; Glycogen synthase kinase 3 beta interacting protein; Centrosome Marker; Glycogen synthase kinase 3 beta interacting protein; GSK3B interacting protein; h Glycogen synthase kinase 3 beta-interacting protein; hNinein; KIAA1565; NIN; NIN protein; NIN_HUMAN; ninein (GSK3B interacting protein); Ninein; Ninein centrosomal protein; SCKL7. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul（10nm 15nm 35nm） |
| 研究领域 | 细胞生物 信号转导 细胞周期蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 243kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Ninein |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存条件 | Store at 2-8 ºC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 产品介绍 |
background: Ninein is a centrosomal protein required for the positioning and anchorage of the microtubule minus-end in epithelial cells. It may also be a centrosome maturation factor and may be involved in microtubule nucleation. Ninein is a component of the core centrosome and is arranged in a tubular shape with an open and a closed end. Function: Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation. Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules. Subunit: Homooligomer. Interacts with GSK3B (GSK3-beta) via its C-terminus domain. Interacts with C14ORF166, such interaction may prevent its phosphorylation by GSK3-beta. Subcellular Location: Cytoplasm, cytoskeleton, centrosome. Tissue Specificity: Ubiquitous. Highly expressed in heart and skeletal muscle. Isoform 1 is more expressed than isoform 5. Post-translational modifications: Phosphorylated by Aurora A (AIK) and PKA kinases but not CK2 or Aurora B (AIM). Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 5 EF-hand domains. Database links: Entrez Gene: 51199 Human Entrez Gene: 18080 Mouse Omim: 608684 Human SwissProt: Q8N4C6 Human SwissProt: Q61043 Mouse Unigene: 310429 Human Unigene: 569387 Human Unigene: 244047 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
