| 产品编号 | bs-7772R-BF555 |
| 英文名称 | Rabbit Anti-SEPT14/BF555 Conjugated antibody |
| 中文名称 | BF555标记的细胞分化蛋白SEPT14抗体 |
| 别 名 | SEPT14; SEPT 14; SEP14_HUMAN; Septin-14; Septin 14; Septin14; FLJ44060. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 细胞周期蛋白 细胞分化 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, ) |
| 产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 50kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SEPT14 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Septin 14, also known as SEPT14, is a 432 amino acid protein that belongs to the septin family. The highly conserved septin family of GTP-binding cytoskeletal proteins is implicated in membrane transport, apoptosis, cell polarity, cell cycle regulation, cytokinesis and other cellular functions. Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. Septin 14 has a GTPase domain followed by a C-terminal coiled-coil domain characteristic of group II septins. Septin 14 interacted with all septins except those that are members of its phylogenetic cluster, including itself. Septin 14 shares highest identity (67%) with Septin 10. Expressed in testis and more weakly in fetal liver, tonsil and thymus, the Septin 14 protein is not detected in testicular cancer or other normal or cancer cell lines. The Septin 14 gene is conserved in chimpanzee, dog, cow, mouse and rat, and maps to human chromosome 7p11.2. Function: SEPT14 is a filament-forming cytoskeletal GTPase. It may play a role in cytokinesis. Subunit: Septins polymerize into heterooligomeric proteincomplexes that form filaments, and can associate with cellularmembranes, actin filaments and microtubules. GTPase activity isrequired for filament formation (By similarity). Interacts withSEPT9. Subcellular Location: Cytoplasm. Tissue Specificity: Testis-specific. Similarity: Belongs to the septin family. Database links: Entrez Gene: 346288 Human Omim: 612140 Human SwissProt: Q6ZU15 Human Unigene: 453629 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. SEPTIN(SEPT)家族是细胞骨架蛋白的一种，具有GTPase的活性。经研究发現，SEPT参与细胞的分裂、细胞膜的运输以及构成细胞骨架。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
