| 产品编号 | bs-0641R-BF488 |
| 英文名称1 | Rabbit Anti-Integrin alpha 4/BF488 Conjugated antibody |
| 中文名称 | BF488标记的整合素α4抗体 |
| 别 名 | Alpha 4 subunit of VLA 4 receptor;ITG-α4: Antigen CD49D; CD 49d ; CD49d; CD49d antigen; CDw49d; IA4; Integrin alpha IV; ITGA 4; ITGA4; LPAM23; MGC90518; Very Late Activation Antigen 4; Very Late Activation Protein 4 Receptor Alpha 4 Subunit; VLA 4; VLA4; integrin alpha-4; ITA4_HUMAN; |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 免疫学 信号转导 干细胞 细胞粘附分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Rat, Cow, Horse, Rabbit, ) |
| 产品应用 | Flow-Cyt=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 111kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Integrin alpha 4 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Integrin alpha 4 (also called CD49d) is a 150 kDa protein that possesses a large extracellular domain involved in ligand binding, a single transmembrane domain, and an intracellular regulatory domain possessing multiple sites for phosphorylation. Integrin alpha 4 forms heterodimers with integrins beta 1 and beta 7. Integrin alpha 4 is expressed on leukocytes and leukocyte precursors, neural crest cells, and developing skeletal muscles and is essential for embryogenesis, hematopoiesis, and immune responses. The presence of integrin alpha 4 promotes cell migration and inhibits cell spreading and contractility. Integrin alpha 4 function has been implicated in the pathogenesis of multiple diseases including asthma, rheumatoid arthritis, Crohn's disease, ulcerative colitis, hepatitis C, and multiple sclerosis, and therefore, modulation of integrin alpha 4 function has become an important target for drug discovery. Function: Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. Subunit: Heterodimer of an alpha and a beta subunit. Subcellular Location: Membrane; Single-pass type I membrane protein. Post-translational modifications: Phosphorylation on Ser-1027 inhibits PXN binding. Similarity: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. Database links: Entrez Gene: 3676 Human Entrez Gene: 16401 Mouse Omim: 192975 Human SwissProt: P13612 Human SwissProt: Q00651 Mouse Unigene: 440955 Human Unigene: 31903 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 整合素-α-4型 Integrin α4 (CD49d)整合素-α是介导细胞与细胞外基质粘附作用的主要因子。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
