产品编号 | bs-6761R-PE-Cy3 |
英文名称1 | Rabbit Anti-IL-10/PE-Cy3 Conjugated antibody |
中文名称 | PE-Cy3标记的白细胞介素-10抗体 |
别 名 | Interleukin-10 precursor; Cytokine synthesis inhibitory factor; CSIF; Cytokine Synthesis Inhibitory Factor; IL 10; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; TGIF; IL10_HUMAN. |
规格价格 | 100ul/2980元 购买 大包装/询价 |
说 明 书 | 100ul |
研究领域 | 细胞生物 免疫学 信号转导 转录调节因子 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, Sheep, ) |
产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 18kDa |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human IL-10 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
产品介绍 |
background: IL-10 protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Subunit: Homodimer. Subcellular Location: Secreted. Tissue Specificity: Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. Similarity: Belongs to the IL-10 family. Database links: Entrez Gene: 3586 Human Entrez Gene: 16153 Mouse Omim: 124092 Human SwissProt: P22301 Human SwissProt: P18893 Mouse Unigene: 193717 Human Unigene: 874 Mouse Unigene: 9868 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 白细胞介素-10(IL-10)是主要由Th2细胞所产生的一种细胞因子,活化的B细胞、单核巨噬细胞和枯否氏细胞(Kupffer's cell)等也可产生IL-10。它是以抑制Th细胞克隆细胞因子合成为特点的多效免疫调节因子。 白介素-10是维护细胞因子网络平衡的重要负调节因子。其作用机制可能是降低抗原递呈细胞MHCⅡ类抗原表达,或诱导抗原递呈细胞产生另一种细胞因子,改变细胞内信号传递途径,从而选择性抑制某些细胞因子mRNA转录,并与Th2细胞产生的IL-4、IL-5有协同作用。 |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |