产品编号 | bs-2221R-Bio |
英文名称1 | Rabbit Anti-ATEXPA10/Biotin Conjugated antibody |
中文名称 | 生物素标记的植物延长蛋白(拟南芥) |
别 名 | ARABIDOPSIS THALIANA EXPANSIN A 10; Alpha-expansin 10; At-EXP10; AtEx10; Ath-ExpAlpha-1.1. |
规格价格 | 100ul/2980元 购买 大包装/询价 |
说 明 书 | 100ul |
产品类型 | 植物抗体 |
研究领域 | 植物 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Arabidopsis Thaliana, ) |
产品应用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 28kDa |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ATEXPA10 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
产品介绍 |
background: structural constituent of cell wall; INVOLVED IN: syncytium formation, plant-type cell wall modification during multidimensional cell growth,unidimensional cell growth, plant-type cell wall loosening; LOCATED IN: plant-type cell wall; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Barwin-related endoglucanase. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 延长蛋白 extensin是存在于植物细胞壁中的羟脯氨酸(Hyp)占有高比例的糖蛋白。细胞壁中含达2—10%,Hyp占构成蛋白质的氨基酸的20%以上,在其他蛋白质中则丝氨酸(Ser)的含量高。由于可以控制植物细胞的伸展(ex-tersion),故称为伸展蛋白。在生长旺盛的幼龄期的细胞中含量少,生长速度迟缓时含量增加,因而认为具有对细胞壁不易伸展的作用。其蛋白质部分具有-ser-Hyp-Hyp-Hyp-Hyp的氨基酸序列,Hyp残基许多有3—4个阿拉伯糖所构成的寡糖;而Ser残基许多具有1—2个半乳糖。脯氨酸羟化是在肽合成后进行,催化此过程的肽基脯氨酸羟化酶需要二价铁离子,可被铁离子络合剂的a′-dipyridyl 所抑制。肽的脯氨酸羟化后,细胞器的糖链结合,被分泌到细胞外,纳入细胞壁中。 |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |