| 产品编号 | bs-2616R-BF647 |
| 英文名称 | Rabbit Anti-IL18R Beta/BF647 Conjugated antibody |
| 中文名称 | BF647标记的白细胞介素-18受体β链抗体 |
| 别 名 | IL-18R Beta; Interleukin-18 receptor beta; Accessory protein like; IL18R Beta; AcPL; IL 18RacP; IL 1R accessor protein like; IL 1R7; IL 1RacPL; IL18Rbeta; Interleukin 18 receptor accessory protein; IL18RAP; Interleukin-18 receptor accessory protein-like; IL-18R-beta; IL-18Rbeta; Accessory protein-like; AcPL; Interleukin-1 receptor 7; IL-1R-7; IL-1R7; CD218 antigen-like family member B; CD218b; I18RA_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, Dog, Horse, ) |
| 产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 68kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IL-18R beta |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: IL18R Beta is an accessory subunit of the heterodimeric receptor for IL18. This protein enhances the IL18 binding activity of IL18R1 (IL1RRP), a ligand binding subunit of IL18 receptor. The coexpression of IL18R1 and IL18R Beta is required for the activation of NF-kappaB and MAPK8 (JNK) in response to IL18. Function: Required for the high affinity binding of interleukin 18 (IL-18) to its receptor complex. Together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK. Subunit: Together with IL18R1, probably belongs to the IL-18 receptor complex. Seems not to interact directly with IL-18. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Strongly expressed in peripheral blood leukocytes, spleen, lung, and, to a lesser extent, in colon, but not in any other tissue tested. Strongly expressed in T-cells polarized with IL-12. Specifically coexpressed with IL18R1 in Th1 cells. Similarity: Belongs to the interleukin-1 receptor family. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 TIR domain. Database links: Entrez Gene: 8807 Human Entrez Gene: 16174 Mouse Omim: 604509 Human SwissProt: O95256 Human SwissProt: Q9Z2B1 Mouse Unigene: 158315 Human Unigene: 20466 Mouse Unigene: 392521 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |
Blank control: K562.
Primary Antibody (green line): Rabbit Anti-IL18R Beta antibody (bs-2616R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
