| 产品编号 | bs-11306R-BF594 |
| 英文名称1 | Rabbit Anti-PCYT1A /BF594 Conjugated antibody |
| 中文名称 | BF594标记的磷酸乙醇胺转移酶A抗体 |
| 别 名 | CCT A; CCT alpha; CCT-alpha; Choline phosphate cytidylyltransferase A; Choline-phosphate cytidylyltransferase A; CT A; CT antibody CTP:phosphocholine cytidylyltransferase A; CTPCT; PCY1A_HUMAN; PCYT1; Pcyt1a; Phosphate cytidylyltransferase 1 choline alpha; Phosphate cytidylyltransferase 1 choline alpha isoform; Phosphorylcholine transferase A. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 神经生物学 信号转导 生长因子和激素 Alzheimer's |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 42kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PCYT1A |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Increase in fetal surfactant synthesis and lung maturity is caused by the glucocorticoidal induction of enzymes required for phosphatidylcholine synthesis towards the end of gestation (1). The regulation of gestational age-dependent induction of phosphatidylcholine synthesis by glucocorticoids is still unclear (1). The rate-controlling enzyme in the phosphatidylcholine biosynthetic pathway is CTP-phosphocholine cytidylyltransferase A (CCT A) (2–4). In cultured eukaryotic cells, this enzyme is essential for survival (3). The alpha isoform is located in the nucleus and is regulated by reversible phosphorylation and membrane association (3). There is significant identity between the alpha-helical membrane-binding domains of CCT A and soybean oleosin (2). Expressed CCT A has lipid-dependent cytidylyltransferase activity (5). The gene which encodes CCT A maps to human chromosome 3q (4). Function: Controls phosphatidylcholine synthesis. Subunit: Homodimer. Subcellular Location: Cytoplasm; cytosol. Membrane. It can interconvert between an inactive cytosolic form and an active membrane-bound form. Post-translational modifications: The serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation. Similarity: Belongs to the cytidylyltransferase family. Database links: Entrez Gene: 5130 Human Entrez Gene: 13026 Mouse Omim: 123695 Human SwissProt: P49585 Human SwissProt: P49586 Mouse Unigene: 135997 Human Unigene: 98775 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
