| 产品编号 | bs-0526R-RBITC |
| 英文名称1 | Rabbit Anti-ALOX5/5 lipoxygenase/RBITC Conjugated antibody |
| 中文名称 | 罗丹明（RBITC）标记的5-脂氧合酶抗体 |
| 别 名 | 5-lipoxygenase; 5-LOX; 5 LO; 5LPG; ALOX 5; ALOX5; Arachidonate 5 lipoxygenase; Arachidonic acid 5 lipoxygenase; Leukotriene A4 synthase; LOG 5; LOG5; MGC163204; LOX5_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 免疫学 转录调节因子 激酶和磷酸酶 合成与降解 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 78kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human 5 Lipoxygenase |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. Function: Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes. Subunit: Interacts with ALOX5AP and LTC4S. Subcellular Location: Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Post-translational modifications: Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus. Similarity: Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain. Database links: Entrez Gene: 240 Human Entrez Gene: 11689 Mouse Omim: 152390 Human SwissProt: P09917 Human SwissProt: P48999 Mouse Unigene: 89499 Human Unigene: 41072 Mouse Unigene: 9662 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 合成与降解（Synthesis and Degradation） 5-脂氧合酶蛋白是花生四烯酸代谢途径中的一种关键酶，在恶性肿瘤的发生、发展过程中起了重要作用。抑制5-脂氧合酶及其产物的表达有可能预防和逆转恶性肿瘤的发生。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
