| 产品编号 | bs-1932R-AP |
| 英文名称 | Rabbit Anti-Vitronectin/AP Conjugated antibody |
| 中文名称 | 碱性磷酸酶（AP）标记的玻璃粘连蛋白抗体 |
| 别 名 | Vitronectin; Complement S Protein; Epibolin; S Protein; Serum Spreading Factor; V75; Vitronectin V10 subunit; VN; VNT; VTN; S-protein; Serum-spreading factor; V75; Vitronectin V10 subunit; VTNC_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 心血管 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, Dog, Cow, ) |
| 产品应用 | IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 9/52kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Vitronectin |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Vitronectin (also known as serum spreading factor, S protein of complement or epibolin) is one of the major multifunctional cell adhesive glycoproteins in mammalian plasma and serum. It is a monomeric acidic glycoprotein detected as a mixture of 75 kD and 65 kD polypeptides. Vitronectin binds to heparin, collagen, streptococci and variety of cultured cells. It also acts as an inhibitor of the complement cascade by binding to the C5b9 complex. Vitronectin promotes cell adhesion and spreading by binding through its cell attachment tripeptide Arg-Gly-Asp (RG-D), activity which is mediated by several different integrin receptors. Apart from the significance for identifying the molecule in the above situations, it also plays an important role in events such as embryonal development, deposition of vitronectin in a number of fibrotic disease states, carcinomas and metastases. Function: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway (By similarity). Subunit: Monomer. Interacts with SERPINE1/PAI1 (By similarity). Subcellular Location: Secreted, extracellular space. Tissue Specificity: Plasma. Post-translational modifications: Sulfated on 2 tyrosine residues (By similarity). N- and O-glycosylated (By similarity). It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both. Similarity: Contains 4 hemopexin-like domains. Contains 1 SMB (somatomedin-B) domain. Database links: Entrez Gene: 7448 Human Entrez Gene: 22370 Mouse Omim: 193190 Human SwissProt: P04004 Human SwissProt: P29788 Mouse Unigene: 2257 Human Unigene: 3667 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
