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Rabbit Anti-GLI1/PE Conjugated antibody (bs-1206R-PE) ~~~促销,代码KX240301~~~
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说 明 书: 100ul  
100ul/2980.00元
大包装/询价

产品编号 bs-1206R-PE
英文名称 Anti-GLI1/PE
中文名称 PE标记的脑胶质瘤相关蛋白抗体(锌指蛋白5)
别    名 Gli 1; Gli1; Gli-1; GLI; GLI Kruppel family member 1; Glioma associated oncogene; Glioma associated oncogene homolog 1 (zinc finger protein); Oncogene GLI; Zfp 5; Zfp5; Zinc finger protein GLI 1; Zinc finger protein GLI1; GLI1_HUMAN.  
规格价格 100ul/2980元 购买        大包装/询价
说 明 书 100ul  
研究领域 肿瘤  细胞生物  神经生物学  信号转导  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Dog, Cow, Horse, )
产品应用 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 118kDa
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GLI1/Zfp5
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品介绍 background:
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Function:
Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.

Subcellular Location:
Cytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.

Tissue Specificity:
Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract.

Database links:

Entrez Gene: 2735 Human

Entrez Gene: 14632 Mouse

Entrez Gene: 140589 Rat

Entrez Gene: 517588 Cow

Omim: 165220 Human

SwissProt: P08151 Human

SwissProt: P47806 Mouse

Unigene: 632702 Human

Unigene: 391450 Mouse

Unigene: 219157 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

GLI1是一种具有很强活性的转录激活因子,GLI1诱导G1/S细胞周期调节蛋白的表达从而促进细胞的增殖; 直接诱导抗凋亡因子Bcl-2的表达以抑制凋亡; 直接激活促进上皮组织向间质转化因子的转录从而加重了肿瘤的侵袭性。目前主要用于肿瘤及神经方面的研究。
产品图片
Blank control: A549.
Primary Antibody (green line): Rabbit Anti-GLI1 antibody (bs-1206R-PE)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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