| 产品编号 | bs-1537R-Bio |
| 英文名称1 | Rabbit Anti-NADPH/Biotin Conjugated antibody |
| 中文名称 | 生物素标记的还原型辅酶Ⅱ/磷酸酰胺腺嘌呤二核苷酸抗体 |
| 别 名 | nicotinamide adenine dinucleotide phosphate; NADP; NADPH; TPNH; Triphosphopyridine nucleotide, reduced form; |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 小分子抗体 |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | |
| 产品应用 | ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 0.833kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated NADPH |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: NDAPH （nicotinamide adenine dinucleotide phosphate) includes of Alpha-NADPH and Beta-NADPH. Beta-NADPH hase activity only. Beta-Nicotinamide adenine dinucleotide 2'-phosphate reduced tetrasodium salt or triphosphopyridine nucleotide reduced tetrasodium salt, also known as Coenzyme Ⅱ. β-Nicotinamide adenine dinucleotide 2′-phosphate (NADP+) and β-Nicotinamide adenine dinucleotide 2′-phosphate, reduced (NADPH) comprise a coenzyme redox pair (NADP+:NADPH) involved in a wide range of enzyme catalyzed oxidation reduction reactions. The NADP+/NADPH redox pair facilitates electron transfer in anabolic reactions such as lipid and cholesterol biosynthesis and fatty acyl chain elongation. The NADP+/NADPH redox pair is used in a variety of antioxidation mechanism where it protects agains reactive oxidation species accumulation. NADPH is generated in vivio by the pentose phosphate pathway (PPP). Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. NADPH氧化酶广泛存在于机体多个系统发挥重要的生理功能。在多种病理情况下，NADPH氧化酶可以在很多组织中催化合成大量活性氧，导致过度氧化应激，使组织细胞的功能下降。 NADPH氧化酶也是血管内皮细胞ROS产生的主要酶系,由其介导产生ROS在信息传递、基因转录、细胞生长和凋亡以及调节内皮依赖性的一氧化氮合酶脱偶联等血管舒张中的作用,使其成为血管生物学中重要的部分,NADPH氧化酶一方面与血管舒张的机能异常有关，另一方面又影响到内皮细胞的结构、粘附分子的表达、渗透、成长及迁移等功能。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
