产品编号 | bs-0917R-APC |
英文名称1 | Rabbit Anti-BrdU/APC Conjugated antibody |
中文名称 | APC标记的5-溴脱氧尿嘧啶核苷抗体 |
别 名 | Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine. |
规格价格 | 100ul/2980元 购买 大包装/询价 |
说 明 书 | 100ul |
产品类型 | 小分子抗体 |
研究领域 | 细胞生物 发育生物学 染色质和核信号 神经生物学 细胞凋亡 细胞周期蛋白 药物及化合物 细胞类型标志物 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Rat, |
产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 0.3071kDa |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | bromodeoxyuridine conjugated to KLH |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
产品介绍 |
background: Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. Anti-bromodeoxyuridine monoclonal antibody has been used for identi-fying proliferating cells in blood (Campana et al., 1988), tissues (Schutte et al., 1987; Hayashi, et al., 1988), tumors (Hoshino et al., 1986; Morstyn et al., 1983), as well as for determining plasma cell labeling indices (Greipp et al., 1985). Subcellular Location: Nuclear. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. BrdU(溴化脱氧尿嘧啶核苷)可以在体内和体外掺入到处于S期的细胞所合成的DNA链中。此抗体可以与掺入到任何种属细胞中的BrdU反应,与碘脱氧尿嘧啶有交叉反应,标记掺有BrdU的S期细胞,主要用于研究各种不同的因素对正常/肿瘤组织的细胞增殖及动力学的研究。 用FITC标记的抗BrdU IgG可用于流式细胞术定量检测增值细胞。 |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |