| 产品编号 | bs-0922R-BF350 |
| 英文名称 | Rabbit Anti-Neurogenin 3/BF350 Conjugated antibody |
| 中文名称 | BF350标记的神经元素3抗体 |
| 别 名 | NGN3; neurogenin 3; Neurog3; Neurogenin3; Neurogenin-3; ATH4B; Atoh5; Math4B; NEUROG3; ngn3; OTTHUMP00000019730; Protein atonal homolog 5. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 神经生物学 信号转导 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Cow, Sheep, ) |
| 产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 23kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NGN3 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Neurogenin-3 (NEUROG3) is expressed in endocrine progenitor cells and is required for endocrine cell development in the pancreas and intestine (Wang et al., 2006 [PubMed 16855267]). It belongs to a family of basic helix-loop-helix transcription factors involved in the determination of neural precursor cells in the neuroectoderm. Function: Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types. Subcellular Location: Nucleus. DISEASE: Defects in NEUROG3 are the cause of diarrhea type 4 (DIAR4) [MIM:610370]. DIAR4 is a characterized by severe, life-threatening watery diarrhea associated with generalized malabsorption and a paucity of enteroendocrine cells. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. Database links: Entrez Gene: 50674 Human Entrez Gene: 11925 Mouse Omim: 604882 Human SwissProt: Q9Y4Z2 Human SwissProt: P70661 Mouse Unigene: 532682 Human Unigene: 57236 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 神经生物学相关蛋白（Neurobiology） Ngn3属于bHLH家族蛋白，是胰腺发育过程中短暂表达的蛋白，可能是胰腺内分泌细胞系的定向因子，一般在成熟胰岛中不存在。 Ngn3的表达会导致胰腺内分泌细胞数的增加外分泌细胞数的减少。因而胰岛β细胞并不是完全靠干细胞分化产生，主要是通过已分化胰岛β细胞的分裂增殖。 但近年来，有学者认为：Ngn3虽在胰腺发育中短暂表达，但是否存在使胰岛干细胞或前体细胞自我更新的能力尚未明确定论。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
