| 产品编号 | bs-6501R-Cy7 |
| 英文名称 | Rabbit Anti-Rab25/Cy7 Conjugated antibody |
| 中文名称 | Cy7标记的癌基因RAS相关蛋白Rab25抗体 |
| 别 名 | CATX 8; CATX-8; CATX8; member RAS oncogene family; RAB25; RAB-25; RAB25_HUMAN; Ras related protein Rab 25; Ras-related protein Rab-25. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 信号转导 转录调节因子 肿瘤细胞生物标志物 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 23kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Rab25 (145-190aa) |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: RAB proteins, such as RAB25, are members of the RASsuperfamily of small GTPases that are involved in membranetrafficking. Members of the RAB11 subfamily, including RAB25,control the return of internalized membrane-associated moieties tothe cell surface Function: Involved in the regulation of cell survival. Promotesinvasive migration of cells in which it functions to localize andmaintain integrin alpha-V/beta-1 at the tips of extendingpseudopodia. Increases the rates of ovarian and breast cancersprogression and aggressiveness by modulating cellular processessuch as proliferation, survival and migration of epithelial tumorcells. May selectively regulate the apical recycling pathway.Together with MYO5B regulates transcytosis Subunit: Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 andRAB11FIP4. Interacts (via the hypervariable C-terminal region) withITGB1 (via the cytoplasmic region); the interaction isGTP-dependent. Interacts with ITGAV. Associates with the integrinalpha-V/beta-1 heterodimer. Subcellular Location: Cell membrane; Lipid-anchor; Cytoplasmicside (Potential). Cell projection, pseudopodium membrane.Cytoplasmic vesicle. Note=Colocalizes with integrin alpha-V/beta-1in vesicles at the pseudopodial tips. Tissue Specificity: Expressed in ovarian epithelium (NOE) andbreast tissue. Expressed in ovarian cancer; expression is increasedrelative to NOE cells. Expression in ovarian cancer is stagedependent, with stage III and stage IV showing higher levels thanearly stage cancers. Expressed in breast cancer; expression isincreased relative to normal breast tissue. Similarity: Belongs to the small GTPase superfamily. Rab family. Database links: Entrez Gene: 57111 Human Entrez Gene: 53868 Mouse Omim: 612942 Human SwissProt: P57735 Human SwissProt: Q9WTL2 Mouse SwissProt: P46629 Rabbit Unigene: 632469 Human Unigene: 26994 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
