| 产品编号 | bs-1308R-FITC |
| 英文名称 | Rabbit Anti-FBXO39/FITC Conjugated antibody |
| 中文名称 | FITC标记的XIAP相关因子1凋亡抑制蛋白抗体 |
| 别 名 | XIAP-associated factor 1; BIRC 4 binding protein; BIRC4-binding protein; XAF1; XAF1_MOUSE; XIAPAF1; BIRC 4 binding protein; BIRC4 binding protein; BIRC4BP; HSXIAPAF 1; HSXIAPAF1; XAF 1; XIAP associated factor 1; Fbxo39; RP24-450M24.4. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 免疫学 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, |
| 产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 31kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse XAF1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: XAF1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF1 results in the neutralization of XIAP's ability to inhibit cell death. XAF1 is normally expressed in all adult and fetal tissues but is present at very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Function: Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells (By similarity). Subunit: Interacts with BIRC1, BIRC2, BIRC3, BIRC4, BIRC7 and BIRC8. Part of an complex consisting of BIRC4, XAF1 and BIRC5; the complex formation requires IFN-beta stimulation (By similarity). Subcellular Location: Cytoplasm (By similarity). Nucleus (By similarity). Mitochondrion (By similarity). Similarity: Contains 1 TRAF-type zinc finger. Database links: Entrez Gene: 628100 Mouse Entrez Gene: 162517 Human Omim: 609106 Human SwissProt: Q8N4B4 Human SwissProt: Q5NBU5 Mouse Unigene: 368364 Human Unigene: 390365 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. XAF1是一个新近发现的通过与XIAP直接结合拮抗其抗凋亡作用的蛋白因子。XAF1是一个新的肿瘤抑制基因，XAF1在多种肿瘤细胞和组织中存在低表达或表达缺失。其过度表达可诱导凋亡，抑制肿瘤生长。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
