| 产品编号 | IHC0507H |
| 英文名称 | Human HSF1 Ready-To-Use IHC Kit |
| 中文名称 | 人热休克因子1即用型免疫组化试剂盒 |
| 别 名 | Heat shock factor 1; Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; hsf1; HSF1_HUMAN; HSTF 1; HSF1; HSTF1 |
| 适用样品基质 | FFPE tissue |
| 保存条件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 产品介绍 |
All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes. SWISS: Q00613 Gene ID: 3297 |
| 产品图片 |
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue slide using IHC0507H (Human HSF1 Kit).
Immunohistochemical analysis of paraffin embedded Human ovarian cancer tissue slide using IHC0507H (Human HSF1 Kit).
Immunohistochemical analysis of paraffin embedded Human colon cancer tissue slide using IHC0507H (Human HSF1 Kit).
Immunohistochemical analysis of paraffin embedded Human testis tissue slide using IHC0507H (Human HSF1 Kit).
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
