产品编号 | bsm-52542R |
英文名称 | MTCO2 Recombinant Rabbit mAb |
中文名称 | 细胞色素c氧化酶亚型2重组兔单抗 |
别 名 | COX2_HUMAN; Cytochrome c oxidase subunit 2; EC:7.1.1.9; Cytochrome c oxidase polypeptide II; mitochondrially encoded cytochrome c oxidase II; MT-CO2; COII; COX2; COXII; |
抗体来源 | Rabbit |
克隆类型 | Recombinant |
克 隆 号 | 6C9 |
交叉反应 | (predicted: Human) |
产品应用 | WB=1:1000-2000,IHC-P=1:200,IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 25 kDa |
检测分子量 | |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human MTCO2 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Contributes to cytochrome-c oxidase activity. Predicted to be involved in mitochondrial electron transport, cytochrome c to oxygen and positive regulation of vasoconstriction. Located in mitochondrial inner membrane. Part of respiratory chain complex IV. Biomarker of Huntington's disease and stomach cancer. [provided by Alliance of Genome Resources, Apr 2022] SWISS: P00403 Gene ID: 4513 细胞色素c氧化酶的组成部分,是线粒体电子传递链中最后一个推动氧化磷酸化的酶。呼吸链包含3个多亚单位复合物琥珀酸脱氢酶(复合体II,CII)、泛醌细胞色素c氧化还原酶(细胞色素b-c1复合物,复合体III,CIII)和细胞色素c氧化酶(复合体IV,CIV),它们协同将NADH和琥珀酸衍生的电子转移到分子氧中,在内膜上形成一个电化学梯度来驱动跨膜转运和ATP合成酶。细胞色素c氧化酶是也是呼吸链的组成部分,催化氧气还原为水。在膜间空间(IMS)中,来自还原细胞色素c的电子通过亚基2的双核铜A中心(CU(A))和 |
产品图片 |
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MTCO2 Monoclonal Antibody, Unconjugated (bsm-52542R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MTCO2 Monoclonal Antibody, Unconjugated (bsm-52542R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MTCO2 Monoclonal Antibody, Unconjugated (bsm-52542R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MTCO2 Monoclonal Antibody, Unconjugated (bsm-52542R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MTCO2 Monoclonal Antibody, Unconjugated (bsm-52542R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MTCO2 Monoclonal Antibody, Unconjugated (bsm-52542R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
The MCF-7 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-MTCO2 antibody (bsm-52542R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |