| 产品编号 | bsm-52542R |
| 英文名称 | MTCO2 Recombinant Rabbit mAb |
| 中文名称 | 细胞色素c氧化酶亚型2重组兔单抗 |
| 别 名 | COX2_HUMAN; Cytochrome c oxidase subunit 2; EC:7.1.1.9; Cytochrome c oxidase polypeptide II; mitochondrially encoded cytochrome c oxidase II; MT-CO2; COII; COX2; COXII; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 6C9 |
| 交叉反应 | (predicted: Human) |
| 产品应用 | WB=1:1000-2000,IHC-P=1:200,IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 25 kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MTCO2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Contributes to cytochrome-c oxidase activity. Predicted to be involved in mitochondrial electron transport, cytochrome c to oxygen and positive regulation of vasoconstriction. Located in mitochondrial inner membrane. Part of respiratory chain complex IV. Biomarker of Huntington's disease and stomach cancer. [provided by Alliance of Genome Resources, Apr 2022] SWISS: P00403 Gene ID: 4513 细胞色素c氧化酶的组成部分，是线粒体电子传递链中最后一个推动氧化磷酸化的酶。呼吸链包含3个多亚单位复合物琥珀酸脱氢酶（复合体II，CII）、泛醌细胞色素c氧化还原酶（细胞色素b-c1复合物，复合体III，CIII）和细胞色素c氧化酶（复合体IV，CIV），它们协同将NADH和琥珀酸衍生的电子转移到分子氧中，在内膜上形成一个电化学梯度来驱动跨膜转运和ATP合成酶。细胞色素c氧化酶是也是呼吸链的组成部分，催化氧气还原为水。在膜间空间（IMS）中，来自还原细胞色素c的电子通过亚基2的双核铜A中心（CU（A））和 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MTCO2 Monoclonal Antibody, Unconjugated (bsm-52542R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MTCO2 Monoclonal Antibody, Unconjugated (bsm-52542R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MTCO2 Monoclonal Antibody, Unconjugated (bsm-52542R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MTCO2 Monoclonal Antibody, Unconjugated (bsm-52542R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MTCO2 Monoclonal Antibody, Unconjugated (bsm-52542R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MTCO2 Monoclonal Antibody, Unconjugated (bsm-52542R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
The MCF-7 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-MTCO2 antibody (bsm-52542R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
