| 产品编号 | IHC0485 |
| 英文名称 | PKC gamma Ready-To-Use IHC Kit |
| 中文名称 | 蛋白激酶C-γ即用型免疫组化试剂盒 |
| 别 名 | PKC; PKC-gamma; PKCgamma; PKCI; Prkc; Prkcc; RATPKCI; PKC gamma; PKCC; PKC-gamma; PKCG; PRKCG; Protein kinase C gamma; Protein kinase C gamma polypeptide; Protein kinase C gamma type; SCA 14; SCA14 |
| 适用样品基质 | FFPE tissue |
| 保存条件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 产品介绍 |
The PKC family of serine/threonine kinases, including PRKCG (PKC gamma), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC gamma is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). SWISS: P05129 Gene ID: 5582 |
| 产品图片 |
Immunohistochemical analysis of paraffin embedded mouse brain tissue slide using IHC0485 (PKC gamma IHC Kit).
Immunohistochemical analysis of paraffin embedded human cerebellum tissue slide using IHC0485 (PKC gamma IHC Kit).
Immunohistochemical analysis of paraffin embedded rat brain tissue slide using IHC0485 (PKC gamma IHC Kit).
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
