| 产品编号 | bs-105336P |
| 英文名称 | Recombinant Human KDM4B Protein, N-His |
| 中文名称 | |
| 别 名 | Lysine-specific demethylase 4B; 1.14.11.66; JmjC domain-containing histone demethylation protein 3B; Jumonji domain-containing protein 2B; [histone H3]-trimethyl-L-lysine(9) demethylase 4B; KDM4B; JHDM3B; JMJD2B; KIAA0876 |
| 克 隆 号 | |
| 理论分子量 | 39.54 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Enables histone H3-methyl-lysine-36 demethylase activity and histone H3-methyl-lysine-9 demethylase activity. Involved in histone H3-K36 demethylation and histone H3-K9 demethylation. Located in cytosol and nucleoplasm. Implicated in autosomal dominant non-syndromic intellectual disability; breast cancer; colorectal cancer; malignant peripheral nerve sheath tumor; and stomach cancer. Biomarker of several diseases, including alopecia areata; lung cancer; medulloblastoma; prostate cancer; and stomach cancer. [provided by Alliance of Genome Resources, Apr 2022] SWISS: O94953 Gene ID: 23030 赖氨酸脱甲基酶4B（KDM4B）是一种组蛋白去甲基化酶，它能催化H3K9me3的去甲基化反应，松弛染色质结构和允许转录因子进入，即通过表观遗传方式调节基因表达。组蛋白赖氨酸残基的甲基化状态对于基因组激活和非激活区域的形成是一个主要决定因素，并且在发育期间对于基因组的正确编码发挥关键作用。研究发现，KDM4B可能在 ERα 结合位点删除抑制性组蛋白标记，为重塑染色质的酶和转录因子产生锚定位点，进而促进 ERα 介导的转录。此外，过表达KDM4B通过对H3K9me3的去甲基化会导致LINE-1拷贝数、转座活性 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
