| 产品编号 | bs-105038P |
| 英文名称 | Recombinant Human MRE11 Protein, C-His |
| 中文名称 | |
| 别 名 | HNGS1; Double-strand break repair protein MRE11; MRE11A; Double-strand break repair protein MRE11A; Meiotic recombination 11 homolog A; MRE11 homolog A; MRE11 homolog 1; MRE11; Meiotic recombination 11 homolog 1 |
| 克 隆 号 | |
| 理论分子量 | 81.56 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] SWISS: P49959 Gene ID: 4361 在细胞中，有多种蛋白参与DNA损伤应答，DNA损伤是可引起癌变的细胞变化，这些蛋白在细胞发生损伤后会启动细胞修复过程，帮助受损的细胞恢复正常。 正常情况下，细胞会经历生长、分化和自然死亡的历程。当细胞受到损伤时，如;辐射损伤或是毒物刺激，一种多蛋白复合物参与的步骤将被启动，进行细胞修复工作并激活其他的生物过程。在这过程中，存在一种MRN复合物，由Mre11，Rad50和NBS1蛋白组成，MRN探测DNA损伤（DNA双链是否断裂）的情况。复合物在探测到DNA损伤信号后将把这个信息传递给一种酶，ATM |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
