| 产品编号 | bs-104967P |
| 英文名称 | Recombinant Human ESR1/ER-alpha Protein, N-His |
| 中文名称 | |
| 别 名 | Estrogen receptor; ER-alpha; ESR; ER; ESR1; Nuclear receptor subfamily 3 group A member 1; NR3A1; Estradiol receptor |
| 克 隆 号 | |
| 理论分子量 | 22.07 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Estrogen and progesterone receptor are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements(EREs) alters transcription of ERE-containing genes. The carboxy terminal region of the estrgen receptor contains the ligand binding domain, the amino terminus serves as the transactivation domain, and the DNA binding domain is centrally located. Two forms of estrogen receptor have been identified, ER Alpha and ER Beta. ER Alpha and ER Beta have been shown to be differentially activated by various ligands. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (hPR-A and hPR-B), which arise from alternative splicing. In most cells, hPR-B functions as a transcriptional activator of progesterone-responsive gene, whereas hPR-A function as a transcriptional inhibitor of all steroid hormone receptors. SWISS: P03372 Gene ID: 2099 类固醇受体（Steroid Receptors） 雌激素受体ER-α（Estrogen Receptor-α, ERα）是一类位于细胞表面或细胞内的特异性蛋白质。 某些特异的雌激素、雌激素类似物、衍生物、拮抗剂或阻断剂与它结合后可引发或阻断受体蛋白质的转化作用。并将雌激素-受体复合物从细胞质再定位进入细胞核以启动蛋白质的生物合成。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
