| 产品编号 | bs-104786P |
| 英文名称 | Recombinant Human CTNNA1 Protein, N-His |
| 中文名称 | |
| 别 名 | Renal carcinoma antigen NY-REN-13; Catenin alpha-1; CTNNA1; Alpha E-catenin; Cadherin-associated protein |
| 克 隆 号 | |
| 理论分子量 | 21.97 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
The distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kDa) found in many tissues bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 proto-oncogene. They are good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. Absence of alpha-catenin is found in certain tumor cell lines and reduced levels in certain human carcinomas. Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. It binds to the N-terminus of alpha-catenin and interacts with the protein product of the tumor suppressor gene APC. This interaction involves a 15-aa repeat in the APC. Beta-catenin cell levels seem to be controlled by APC. The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR. SWISS: P35221 Gene ID: 1495 细胞粘附蛋白（Call Adhesion Protein） α-catenin是一种多功能的蛋白质，能链接E-\N-\P-钙粘附分子,具有参与细胞粘附和介导信号转导的双重功能，并与肿瘤的发生发展及浸润密切相关。 在正常个体中，βα-catenin和钙黏蛋白形成复合体，介导同型细胞的粘附，维持细胞的稳定；同时，α-catenin作为Wnt/α-catenin信号通路的关键成员在介导信号转导过程中调控细胞的增殖和凋亡。 在恶性肿瘤中，α连环蛋白的表达呈现明显的异质性，促使细胞异常增殖，还可使细胞之间的黏附性减 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
