| 产品编号 | bs-104755P |
| 英文名称 | Recombinant Human PPP1R12A Protein, N-His |
| 中文名称 | |
| 别 名 | MYPT1; Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; MBS; PPP1R12A; Protein phosphatase myosin-binding subunit; Myosin phosphatase target subunit 1 |
| 克 隆 号 | |
| 理论分子量 | 35.33 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in non muscle cells. The guanosine triphosphate (GTP) bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho associated kinase (Rho kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Therefore Rho appears to inhibit myosin phosphatase through the action of Rho kinase. SWISS: O14974 Gene ID: 4659 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
