| 产品编号 | bs-104154P |
| 英文名称 | Recombinant Human PRMT5 Protein, N-His |
| 中文名称 | |
| 别 名 | PRMT5; SKB1 homolog; 72 kDa ICln-binding protein; Jak-binding protein 1; Histone-arginine N-methyltransferase PRMT5; SKB1Hs; JBP1; Shk1 kinase-binding protein 1 homolog; SKB1; HRMT1L5; IBP72; Protein arginine N-methyltransferase 5 |
| 克 隆 号 | |
| 理论分子量 | 21.71 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] SWISS: O14744 Gene ID: 10419 精氨酸甲基转移酶，既能催化omega-N单甲基精氨酸（MMA）和对称二甲基精氨酸（sDMA）的形成，又能优先催化MMA的形成。特异性介导小核核糖核蛋白smd1（SNRPD1）和smd3（SNRPD3）中精氨酸残基的对称二甲基化；这种甲基化是snRNP核心粒子组装和生物生成所必需的。甲基化SUPT5H并可能调节其转录延伸特性。髓鞘碱性蛋白（MBP）精氨酸残基的单酸和二甲基化。可能在细胞因子激活的转导途径中发挥作用。负调控细胞周期素E1启动子活性和细胞增殖。在生殖细胞发育过程中甲基化组蛋白H2A和H4'Arg |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
