| 产品编号 | bs-104087P |
| 英文名称 | Recombinant Human MSH2 Protein, N-His |
| 中文名称 | |
| 别 名 | hMSH2; MutS protein homolog 2; MSH2; DNA mismatch repair protein Msh2 |
| 克 隆 号 | |
| 理论分子量 | 33.75 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis. SWISS: P43246 Gene ID: 4436 MSH2是核苷酸错配修复酶之一，其突变和多种肿瘤的发生有关，主要用于：先天性结肠癌、散发性结肠癌及一些消化系统肿瘤方面的研究。DNA错配修复系统是指由特异修复DNA碱基错配的酶组成，保证DNA遗传物质的高保真性。它由MLH1、MSH2、PMS1、PMS2、MSH6和MSH3基因组成。如果上述基因发生突变或失活，会使细胞错配修复功能缺陷，产生遗传不稳定性，导致肿瘤易感。MSH2基因突变发生在散发性结肠癌中。 hMSH-2和hMLH1都与肿瘤的发生有关。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
