| 产品编号 | bs-104014P |
| 英文名称 | Recombinant Human LIMK1 Protein, N-His |
| 中文名称 | |
| 别 名 | LIMK-1; LIMK; LIMK1; LIM domain kinase 1 |
| 克 隆 号 | |
| 理论分子量 | 28.59 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. LIMK1 may be involved in brain development; it is highly expressed in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle. SWISS: P53667 Gene ID: 3984 LIM激酶1(LIMkinase1,LIMK-1)是单丝氨酸蛋白激酶,LIMK-1的活化受多种机制调控,成为联系细胞外刺激与细胞骨架稳定性的枢纽,在多种基本的生物学过程中起重要作用。LIMK-1包含LIM和PDZ蛋白和蛋白相互作用区、P/S结构域、激酶结构域,在神经元中呈高表达。它的主要作用是使肌动蛋白解聚因子cofilin磷酸化和失活,从而导致肌动蛋白细胞骨架的改变。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
