| 产品编号 | bs-103944P |
| 英文名称 | Recombinant Human DIABLO Protein, N-His |
| 中文名称 | |
| 别 名 | Second mitochondria-derived activator of caspase; Diablo homolog; mitochondrial; DIABLO; Smac; SMAC; Direct IAP-binding protein with low pI |
| 克 隆 号 | |
| 理论分子量 | 22.04 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
bs-1298P is one synthetic peptide derived from human Smac. This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Several alternatively spliced transcript variants that encode distinct isoforms have been described for this gene but the validity of some transcripts, and their predicted ORFs, has not been determined conclusively. The inhibitor of apoptosis (IAP) proteins regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes their inhibitory effect on caspases has been designated Smac/DIABLO. This is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates the cytochrome c/Apaf-1/caspase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues. SWISS: Q9NR28 Gene ID: 56616 Smac（second mitochondria-derived activator of caspase）又称DIABLO（direct IAP binding protein），是一种线粒体蛋白，也是重要的促细胞凋亡蛋白之一，能够促进细胞凋亡，当杀死不正常细胞的机制启动后，线粒体释放出Smac蛋白促使受损或不正常细胞凋亡的生化过程就会出现，Smac在诱导肿瘤细胞凋亡，增加肿瘤细胞的化疗敏感性及增强免疫力等方面有一定的意义和应用前景。Smac是第二个线粒体来源的胱氨酸酶激活剂，它通过拮抗或中和一种或几种 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
